Magnetic Bead Immunoassay (MIA) 2.3.1. (Ar-C), 133.8 (Ar-C), 131.3 (Ar-C), 131.3 (Ar-C), 131.0 (Ar-C), 129.3 (Ar-C), 115.1 (Ar-C), 113.4 (Ar-C), 107.4 (Ar-C), 96.4 (Ar-C), 78.5 (C), 77.8 (CH), 53.3 (CH2), 45.8 (CH2), 12.9 (CH3). 2.2.2. 4-(2-Amino-phenyl)-2-tert-butoxycarbonylamino-4-oxo-butyric Acidity 2 NH2 security of kynurenine was completed by Boc-chemistry pursuing SchottenCBaumann circumstances: kynurenine (500 mg, 2.4 mmol, 1 eq) was dissolved within a drinking water/THF 1:1 mixture containing NaOH (288 mg, 7.2 mmol, 3 eq). Following the alternative turned apparent, Boc2O (1.55 mL, 7.2 mmol, 3 eq) was added dropwise at 0 C, as well as the response was monitored by TLC. Comprehensive conversion was noticed after 90 min. Acidification Alloxazine using 10% HCl, accompanied by 3 extractions with ethyl acetate, drying out from the organic level and evaporation from the solvent yielded the crude item: a yellowish essential oil. Column chromatography (ethyl acetate) yielded the 100 % pure item: a yellowish natural powder (327.7 mg, 44%). 1H-NMR (400 MHz, CDCl3): 7.73 (d, = 8.0 Hz, 1H- Ar-H), 7.34 (t, = 7.6 Hz, 1H, Ar-H), 6.83C6.72 (m, 2H, Ar-H), 5.64 (d, = 8.7 Hz, 1H, CH), 4.67 (ddd, = 12.8, 8.5, 4.3 Hz, 1H, CH), 3.80C3.69 (m, 2H, CH2), 3.53 (dd, = 18.0, 3.8 Hz, 1H, CH), 1.45 (s, 9H, 3x CH3). 13C-NMR (100 MHz, CDCl3): 199.54 (Ar-C(=O)), 172.43 (C(C=O)OH), 155.68 (Boc-C(=O)), 149.45 (Ar-C), 134.96 (Ar-C), 131.08 (Ar-C), 125.83 (Ar-C), 118.10 (Ar-C), 116.73 (Ar-C), 69.98 (Boc-= 7.9, 1.0 Hz, 1H, Ar-H), 7.91C7.85 (m, 3H, Ar-H), 7.83C7.79 (m, 2H, Ar-H), 7.47 (dd, = 7.6, 0.8 Hz, 1H, Ar-H), 7.08C7.00 (m, 2H, Ar-H), 6.97C6.90 (m, 5H, Ar-H), 5.04 (s, 2H, Ar-CH2), 4.65 (d, Alloxazine = 2.4 Hz, 1H, CH2), 4.44 (t, = 5.2 Hz, 2H, Triazol-CH2), 3.74 (t, = 5.2 Hz, 2H, O-CH2), 3.63 (dd, = 13.9, 6.7 Hz, 8H, 4x RhB-CH2), 3.48C3.45 (m, 2H, O-CH2), 3.44C3.40 (m, 6H, 3 O-CH2), 3.30 (t, = 6.4 Hz, 2H, O-CH2), 3.00 (dd, = 12.0, 6.0 Hz, 2H, CH2), 1.33 (s, 9H, 3 Boc-CH3), 1.20 (t, = 6.7 Hz, 12H, 4x RhB-CH3). 13C-NMR (DMSO, 125 MHz): 198.9 (Ar-C(=O), 172.1 (C(=O)-NH), 164.9 (C(=O)-O), 157.8 (Ar-H), Alloxazine 157.5 (Boc-C(=O)), 156.0 (Ar-C), 155.6 (Ar-C), 155.5 (Ar-C), 141.04 (Triazol-Ar-C), 134.6 (Ar-C), 133.9 (Ar-C), 133.8 (Ar-C), 133.7 (Ar-C), 131.3 (Ar-C), 131.2 (Ar-C), 131.0 LAIR2 (Ar-C), 130.9 (Ar-C), 129.6 (Ar-C), 129.2 (Ar-C), 125.3 (Triazol-Ar-C), 115.0 (Ar-C), 114.9 (Ar-C), 114.8 (Ar-C), 113.3 (Ar-C), 96.3 (Ar-C), 78.4 (= 8.0, 1.4, 1H, Ar-H), 8.11 (s, 1H, C(=O)NH), 7.93C7.84 (m, 3H, Ar-H), 7.85C7.78 (m, 2H, Ar-H), 7.47 (dd, = 7.7, 1.4, 1H, Ar-H), 7.05C6.99 (m, 3H, Ar-H), 6.97C6.89 (m, 5H, Ar-H), 5.05 (d, = 4.4, 3H, Ar-CH2 + CH), 4.47C4.41 (m, 2H, Ar-CH2), 3.75 (t, = 5.2, 2H, O-CH2), 3.63 (q, = 6.9, 8H, RhB-CH2), 3.54 (d, = 5.6, 2H, O-CH2), 3.50C3.42 (m, 8H, O-CH2), 2.92 (q, = 5.6, 2H, O-CH2), 1.20 (t, = 7.0, 12H, RhB-CH3). 13C-NMR (DMSO, 125 MHz): 197.3 (Ar-C(=O)), 168.8 (C(=O)-NH), 164.9 (C(=O)-O), 158.2 (Ar-C), 157.9 (Ar-C), 157.5 (Ar-C), Alloxazine 155.5 (Ar-C), 141.1 (Triazol-Ar-C), 133.2 (Ar-C), 133.7 (Ar-C), 131.2 (Ar-C), 130.9 (Ar-C), 129.6 (Ar-C), 125.3 (Triazol-Ar-C), 117.5 (Ar-C), 114.9 (Ar-C), 113.3 (Ar-C), 96.3 (Ar-C), 70.1 (O-CH2), 70.0 (O-CH2), 69.9 (O-CH2), 69.0 (O-CH2), 67.1 (O-CH2), 58.6 (Ar-CH2), 49.8 (Ar-CH2), 45.7 N-CH2), 12.87 (CH3). 2.2.6. (6-Diethylamino-9-2-[1-(2-2-[2-(2-hydroxy-ethoxy)-ethoxy]-ethoxy-ethyl)-1H-[1,2,3]tr-iazol-4-ylmethoxycarbonyl]-phenyl-xanthen-3-ylidene)-diethyl-ammonium Sodium 6 Propargyl rhodamine B 1 (100 mg, 193.4 mol, 1 eq) and azidotetraethylene glycol (42.4 mg, 193.4 mol, 1 eq, synthesized based on the books [40]) had been dissolved in 25 mL of an assortment of CH2Cl2/MeOH/H2O 10:10:3. A remedy of CuSO4 in H2O (0.5 M, 15.5 L, 7.74 mol, Alloxazine 0.04 eq) was added, TBTA (1 mg, 1.93 mol, 0.01 eq) and Na ascorbate (8.4 mg, 42.6 mol, 0.22 eq) were added, as well as the mix was heated to 60 C. The response was supervised by TLC. After 16 h, the mix was still left to cool off, accompanied by the addition of 25 mL of ddH2O. Removal with 50 mL of CH2Cl2 three times, accompanied by drying out over Na2SO4, provided the crude item. Column chromatography (CH2Cl2/MeOH 3:1) provided the pure item: a red essential oil (135.8 mg, 95%). 1H-NMR (DMSO, 500 MHz): 8.22 (d, = 8.0 Hz, 1H, Ar-H), 7.89 (t, = 5.2 Hz, 2H, Ar-H), 7.82 (t, = 7.9 Hz, 1H, Ar-H), 7.48 (d, = 7.5 Hz, 1H, Ar-H), 7.04 (dd, = 9.5, 2.2 Hz, 2H, Ar-H),.